Transfection:Article Title: Stress Activated NRF2-MDM2 Cascade Controls Neoplastic Progression in Pancreas
Article Snippet: .. MIA PaCa-2 cells seeded in 24-well plates at a 2 × 10 4 cells/well were transfected with Lipofectamine 3000 reagent (Thermo Fisher Scientific, L3000015) with the following plasmids and sh-RNAs: pGL3Basic-Mdm2-T1 (Addgene, Plasmid# 32365) ( Chang et al., 2004 ) and its corresponding ARE mutant plasmid PGL3Basic-Mdm2-ARE-mut obtained by site-directed mutagenesis, pRL-TK control plasmid (Promega, E2241), WT p62 expression vector ( Umemura et al., 2016 ), pCDNA3-Myc3-Nrf2 (Addgene, Plasmid# 21555) ( Furukawa and Xiong, 2005 ) , Flag-p53/pRK5 (Addgene 39237) ( Jiang et al., 2011 ) , NRF2-shRNA (Santa Cruz, sc-37030-SH), Control-shRNA (Santa Cruz, sc-108060) or treated with 10 μM sulforaphane (Millipore Sigma, S4441) or DMSO as control. ..
Article Title: Stress Activated NRF2-MDM2 Cascade Controls Neoplastic Progression in Pancreas
Article Snippet: .. Luciferase Assay MIA PaCa-2 cells seeded in 24-well plates at a 2 × 10 4 cells/well were transfected with Lipofectamine 3000 reagent (Thermo Fisher Scientific, L3000015) with the following plasmids and sh-RNAs: pGL3Basic-Mdm2-T1 (Addgene, Plasmid# 32365) ( Chang et al., 2004 ) and its corresponding ARE mutant plasmid PGL3Basic-Mdm2-ARE-mut obtained by site-directed mutagenesis, pRL-TK control plasmid (Promega, E2241), WT p62 expression vector ( Umemura et al., 2016 ), pCDNA3-Myc3-Nrf2 (Addgene, Plasmid# 21555) ( Furukawa and Xiong, 2005 ) , Flag-p53/pRK5 (Addgene 39237) ( Jiang et al., 2011 ) , NRF2-shRNA (Santa Cruz, sc-37030-SH), Control-shRNA (Santa Cruz, sc-108060) or treated with 10 μM sulforaphane (Millipore Sigma, S4441) or DMSO as control. ..
Plasmid Preparation:Article Title: Stress Activated NRF2-MDM2 Cascade Controls Neoplastic Progression in Pancreas
Article Snippet: .. MIA PaCa-2 cells seeded in 24-well plates at a 2 × 10 4 cells/well were transfected with Lipofectamine 3000 reagent (Thermo Fisher Scientific, L3000015) with the following plasmids and sh-RNAs: pGL3Basic-Mdm2-T1 (Addgene, Plasmid# 32365) ( Chang et al., 2004 ) and its corresponding ARE mutant plasmid PGL3Basic-Mdm2-ARE-mut obtained by site-directed mutagenesis, pRL-TK control plasmid (Promega, E2241), WT p62 expression vector ( Umemura et al., 2016 ), pCDNA3-Myc3-Nrf2 (Addgene, Plasmid# 21555) ( Furukawa and Xiong, 2005 ) , Flag-p53/pRK5 (Addgene 39237) ( Jiang et al., 2011 ) , NRF2-shRNA (Santa Cruz, sc-37030-SH), Control-shRNA (Santa Cruz, sc-108060) or treated with 10 μM sulforaphane (Millipore Sigma, S4441) or DMSO as control. ..
Article Title: Stress Activated NRF2-MDM2 Cascade Controls Neoplastic Progression in Pancreas
Article Snippet: .. Site-directed Mutagenesis Mutant promoter construct with mutated ARE (−360 bp ARE; TGACTCAGC) in PGL3Basic-Mdm2-T1 (Addgene, Plasmid# 32365), was generated by PCR amplification using the QuikChange II XL Site-Directed Mutagenesis (Agilent Technologies, #200521) and the primers listed in the Key Resources Table. .. Sequence was verified by GENEWIZ DNA Sequencing Services, using the oligo: GCTAGCGGCGTTCTCTCCGCGGAC.
Article Title: Stress Activated NRF2-MDM2 Cascade Controls Neoplastic Progression in Pancreas
Article Snippet: .. Mutant promoter construct with mutated ARE (−360 bp ARE; TGACTCAGC) in PGL3Basic-Mdm2-T1 (Addgene, Plasmid# 32365), was generated by PCR amplification using the QuikChange II XL Site-Directed Mutagenesis (Agilent Technologies, #200521) and the primers listed in the Key Resources Table. .. Sequence was verified by GENEWIZ DNA Sequencing Services, using the oligo: GCTAGCGGCGTTCTCTCCGCGGAC.
Article Title: Stress Activated NRF2-MDM2 Cascade Controls Neoplastic Progression in Pancreas
Article Snippet: .. Luciferase Assay MIA PaCa-2 cells seeded in 24-well plates at a 2 × 10 4 cells/well were transfected with Lipofectamine 3000 reagent (Thermo Fisher Scientific, L3000015) with the following plasmids and sh-RNAs: pGL3Basic-Mdm2-T1 (Addgene, Plasmid# 32365) ( Chang et al., 2004 ) and its corresponding ARE mutant plasmid PGL3Basic-Mdm2-ARE-mut obtained by site-directed mutagenesis, pRL-TK control plasmid (Promega, E2241), WT p62 expression vector ( Umemura et al., 2016 ), pCDNA3-Myc3-Nrf2 (Addgene, Plasmid# 21555) ( Furukawa and Xiong, 2005 ) , Flag-p53/pRK5 (Addgene 39237) ( Jiang et al., 2011 ) , NRF2-shRNA (Santa Cruz, sc-37030-SH), Control-shRNA (Santa Cruz, sc-108060) or treated with 10 μM sulforaphane (Millipore Sigma, S4441) or DMSO as control. ..
Mutagenesis:Article Title: Stress Activated NRF2-MDM2 Cascade Controls Neoplastic Progression in Pancreas
Article Snippet: .. MIA PaCa-2 cells seeded in 24-well plates at a 2 × 10 4 cells/well were transfected with Lipofectamine 3000 reagent (Thermo Fisher Scientific, L3000015) with the following plasmids and sh-RNAs: pGL3Basic-Mdm2-T1 (Addgene, Plasmid# 32365) ( Chang et al., 2004 ) and its corresponding ARE mutant plasmid PGL3Basic-Mdm2-ARE-mut obtained by site-directed mutagenesis, pRL-TK control plasmid (Promega, E2241), WT p62 expression vector ( Umemura et al., 2016 ), pCDNA3-Myc3-Nrf2 (Addgene, Plasmid# 21555) ( Furukawa and Xiong, 2005 ) , Flag-p53/pRK5 (Addgene 39237) ( Jiang et al., 2011 ) , NRF2-shRNA (Santa Cruz, sc-37030-SH), Control-shRNA (Santa Cruz, sc-108060) or treated with 10 μM sulforaphane (Millipore Sigma, S4441) or DMSO as control. ..
Article Title: Stress Activated NRF2-MDM2 Cascade Controls Neoplastic Progression in Pancreas
Article Snippet: .. Site-directed Mutagenesis Mutant promoter construct with mutated ARE (−360 bp ARE; TGACTCAGC) in PGL3Basic-Mdm2-T1 (Addgene, Plasmid# 32365), was generated by PCR amplification using the QuikChange II XL Site-Directed Mutagenesis (Agilent Technologies, #200521) and the primers listed in the Key Resources Table. .. Sequence was verified by GENEWIZ DNA Sequencing Services, using the oligo: GCTAGCGGCGTTCTCTCCGCGGAC.
Article Title: Stress Activated NRF2-MDM2 Cascade Controls Neoplastic Progression in Pancreas
Article Snippet: .. Mutant promoter construct with mutated ARE (−360 bp ARE; TGACTCAGC) in PGL3Basic-Mdm2-T1 (Addgene, Plasmid# 32365), was generated by PCR amplification using the QuikChange II XL Site-Directed Mutagenesis (Agilent Technologies, #200521) and the primers listed in the Key Resources Table. .. Sequence was verified by GENEWIZ DNA Sequencing Services, using the oligo: GCTAGCGGCGTTCTCTCCGCGGAC.
Article Title: Stress Activated NRF2-MDM2 Cascade Controls Neoplastic Progression in Pancreas
Article Snippet: .. Luciferase Assay MIA PaCa-2 cells seeded in 24-well plates at a 2 × 10 4 cells/well were transfected with Lipofectamine 3000 reagent (Thermo Fisher Scientific, L3000015) with the following plasmids and sh-RNAs: pGL3Basic-Mdm2-T1 (Addgene, Plasmid# 32365) ( Chang et al., 2004 ) and its corresponding ARE mutant plasmid PGL3Basic-Mdm2-ARE-mut obtained by site-directed mutagenesis, pRL-TK control plasmid (Promega, E2241), WT p62 expression vector ( Umemura et al., 2016 ), pCDNA3-Myc3-Nrf2 (Addgene, Plasmid# 21555) ( Furukawa and Xiong, 2005 ) , Flag-p53/pRK5 (Addgene 39237) ( Jiang et al., 2011 ) , NRF2-shRNA (Santa Cruz, sc-37030-SH), Control-shRNA (Santa Cruz, sc-108060) or treated with 10 μM sulforaphane (Millipore Sigma, S4441) or DMSO as control. ..
Control:Article Title: Stress Activated NRF2-MDM2 Cascade Controls Neoplastic Progression in Pancreas
Article Snippet: .. MIA PaCa-2 cells seeded in 24-well plates at a 2 × 10 4 cells/well were transfected with Lipofectamine 3000 reagent (Thermo Fisher Scientific, L3000015) with the following plasmids and sh-RNAs: pGL3Basic-Mdm2-T1 (Addgene, Plasmid# 32365) ( Chang et al., 2004 ) and its corresponding ARE mutant plasmid PGL3Basic-Mdm2-ARE-mut obtained by site-directed mutagenesis, pRL-TK control plasmid (Promega, E2241), WT p62 expression vector ( Umemura et al., 2016 ), pCDNA3-Myc3-Nrf2 (Addgene, Plasmid# 21555) ( Furukawa and Xiong, 2005 ) , Flag-p53/pRK5 (Addgene 39237) ( Jiang et al., 2011 ) , NRF2-shRNA (Santa Cruz, sc-37030-SH), Control-shRNA (Santa Cruz, sc-108060) or treated with 10 μM sulforaphane (Millipore Sigma, S4441) or DMSO as control. ..
Article Title: Stress Activated NRF2-MDM2 Cascade Controls Neoplastic Progression in Pancreas
Article Snippet: .. Luciferase Assay MIA PaCa-2 cells seeded in 24-well plates at a 2 × 10 4 cells/well were transfected with Lipofectamine 3000 reagent (Thermo Fisher Scientific, L3000015) with the following plasmids and sh-RNAs: pGL3Basic-Mdm2-T1 (Addgene, Plasmid# 32365) ( Chang et al., 2004 ) and its corresponding ARE mutant plasmid PGL3Basic-Mdm2-ARE-mut obtained by site-directed mutagenesis, pRL-TK control plasmid (Promega, E2241), WT p62 expression vector ( Umemura et al., 2016 ), pCDNA3-Myc3-Nrf2 (Addgene, Plasmid# 21555) ( Furukawa and Xiong, 2005 ) , Flag-p53/pRK5 (Addgene 39237) ( Jiang et al., 2011 ) , NRF2-shRNA (Santa Cruz, sc-37030-SH), Control-shRNA (Santa Cruz, sc-108060) or treated with 10 μM sulforaphane (Millipore Sigma, S4441) or DMSO as control. ..
Expressing:Article Title: Stress Activated NRF2-MDM2 Cascade Controls Neoplastic Progression in Pancreas
Article Snippet: .. MIA PaCa-2 cells seeded in 24-well plates at a 2 × 10 4 cells/well were transfected with Lipofectamine 3000 reagent (Thermo Fisher Scientific, L3000015) with the following plasmids and sh-RNAs: pGL3Basic-Mdm2-T1 (Addgene, Plasmid# 32365) ( Chang et al., 2004 ) and its corresponding ARE mutant plasmid PGL3Basic-Mdm2-ARE-mut obtained by site-directed mutagenesis, pRL-TK control plasmid (Promega, E2241), WT p62 expression vector ( Umemura et al., 2016 ), pCDNA3-Myc3-Nrf2 (Addgene, Plasmid# 21555) ( Furukawa and Xiong, 2005 ) , Flag-p53/pRK5 (Addgene 39237) ( Jiang et al., 2011 ) , NRF2-shRNA (Santa Cruz, sc-37030-SH), Control-shRNA (Santa Cruz, sc-108060) or treated with 10 μM sulforaphane (Millipore Sigma, S4441) or DMSO as control. ..
Article Title: Stress Activated NRF2-MDM2 Cascade Controls Neoplastic Progression in Pancreas
Article Snippet: .. Luciferase Assay MIA PaCa-2 cells seeded in 24-well plates at a 2 × 10 4 cells/well were transfected with Lipofectamine 3000 reagent (Thermo Fisher Scientific, L3000015) with the following plasmids and sh-RNAs: pGL3Basic-Mdm2-T1 (Addgene, Plasmid# 32365) ( Chang et al., 2004 ) and its corresponding ARE mutant plasmid PGL3Basic-Mdm2-ARE-mut obtained by site-directed mutagenesis, pRL-TK control plasmid (Promega, E2241), WT p62 expression vector ( Umemura et al., 2016 ), pCDNA3-Myc3-Nrf2 (Addgene, Plasmid# 21555) ( Furukawa and Xiong, 2005 ) , Flag-p53/pRK5 (Addgene 39237) ( Jiang et al., 2011 ) , NRF2-shRNA (Santa Cruz, sc-37030-SH), Control-shRNA (Santa Cruz, sc-108060) or treated with 10 μM sulforaphane (Millipore Sigma, S4441) or DMSO as control. ..
Construct:Article Title: Stress Activated NRF2-MDM2 Cascade Controls Neoplastic Progression in Pancreas
Article Snippet: .. Site-directed Mutagenesis Mutant promoter construct with mutated ARE (−360 bp ARE; TGACTCAGC) in PGL3Basic-Mdm2-T1 (Addgene, Plasmid# 32365), was generated by PCR amplification using the QuikChange II XL Site-Directed Mutagenesis (Agilent Technologies, #200521) and the primers listed in the Key Resources Table. .. Sequence was verified by GENEWIZ DNA Sequencing Services, using the oligo: GCTAGCGGCGTTCTCTCCGCGGAC.
Article Title: Stress Activated NRF2-MDM2 Cascade Controls Neoplastic Progression in Pancreas
Article Snippet: .. Mutant promoter construct with mutated ARE (−360 bp ARE; TGACTCAGC) in PGL3Basic-Mdm2-T1 (Addgene, Plasmid# 32365), was generated by PCR amplification using the QuikChange II XL Site-Directed Mutagenesis (Agilent Technologies, #200521) and the primers listed in the Key Resources Table. .. Sequence was verified by GENEWIZ DNA Sequencing Services, using the oligo: GCTAGCGGCGTTCTCTCCGCGGAC.
Generated:Article Title: Stress Activated NRF2-MDM2 Cascade Controls Neoplastic Progression in Pancreas
Article Snippet: .. Site-directed Mutagenesis Mutant promoter construct with mutated ARE (−360 bp ARE; TGACTCAGC) in PGL3Basic-Mdm2-T1 (Addgene, Plasmid# 32365), was generated by PCR amplification using the QuikChange II XL Site-Directed Mutagenesis (Agilent Technologies, #200521) and the primers listed in the Key Resources Table. .. Sequence was verified by GENEWIZ DNA Sequencing Services, using the oligo: GCTAGCGGCGTTCTCTCCGCGGAC.
Article Title: Stress Activated NRF2-MDM2 Cascade Controls Neoplastic Progression in Pancreas
Article Snippet: .. Mutant promoter construct with mutated ARE (−360 bp ARE; TGACTCAGC) in PGL3Basic-Mdm2-T1 (Addgene, Plasmid# 32365), was generated by PCR amplification using the QuikChange II XL Site-Directed Mutagenesis (Agilent Technologies, #200521) and the primers listed in the Key Resources Table. .. Sequence was verified by GENEWIZ DNA Sequencing Services, using the oligo: GCTAGCGGCGTTCTCTCCGCGGAC.
Polymerase Chain Reaction:Article Title: Stress Activated NRF2-MDM2 Cascade Controls Neoplastic Progression in Pancreas
Article Snippet: .. Site-directed Mutagenesis Mutant promoter construct with mutated ARE (−360 bp ARE; TGACTCAGC) in PGL3Basic-Mdm2-T1 (Addgene, Plasmid# 32365), was generated by PCR amplification using the QuikChange II XL Site-Directed Mutagenesis (Agilent Technologies, #200521) and the primers listed in the Key Resources Table. .. Sequence was verified by GENEWIZ DNA Sequencing Services, using the oligo: GCTAGCGGCGTTCTCTCCGCGGAC.
Article Title: Stress Activated NRF2-MDM2 Cascade Controls Neoplastic Progression in Pancreas
Article Snippet: .. Mutant promoter construct with mutated ARE (−360 bp ARE; TGACTCAGC) in PGL3Basic-Mdm2-T1 (Addgene, Plasmid# 32365), was generated by PCR amplification using the QuikChange II XL Site-Directed Mutagenesis (Agilent Technologies, #200521) and the primers listed in the Key Resources Table. .. Sequence was verified by GENEWIZ DNA Sequencing Services, using the oligo: GCTAGCGGCGTTCTCTCCGCGGAC.
Amplification:Article Title: Stress Activated NRF2-MDM2 Cascade Controls Neoplastic Progression in Pancreas
Article Snippet: .. Site-directed Mutagenesis Mutant promoter construct with mutated ARE (−360 bp ARE; TGACTCAGC) in PGL3Basic-Mdm2-T1 (Addgene, Plasmid# 32365), was generated by PCR amplification using the QuikChange II XL Site-Directed Mutagenesis (Agilent Technologies, #200521) and the primers listed in the Key Resources Table. .. Sequence was verified by GENEWIZ DNA Sequencing Services, using the oligo: GCTAGCGGCGTTCTCTCCGCGGAC.
Article Title: Stress Activated NRF2-MDM2 Cascade Controls Neoplastic Progression in Pancreas
Article Snippet: .. Mutant promoter construct with mutated ARE (−360 bp ARE; TGACTCAGC) in PGL3Basic-Mdm2-T1 (Addgene, Plasmid# 32365), was generated by PCR amplification using the QuikChange II XL Site-Directed Mutagenesis (Agilent Technologies, #200521) and the primers listed in the Key Resources Table. .. Sequence was verified by GENEWIZ DNA Sequencing Services, using the oligo: GCTAGCGGCGTTCTCTCCGCGGAC.
Luciferase:Article Title: Stress Activated NRF2-MDM2 Cascade Controls Neoplastic Progression in Pancreas
Article Snippet: .. Luciferase Assay MIA PaCa-2 cells seeded in 24-well plates at a 2 × 10 4 cells/well were transfected with Lipofectamine 3000 reagent (Thermo Fisher Scientific, L3000015) with the following plasmids and sh-RNAs: pGL3Basic-Mdm2-T1 (Addgene, Plasmid# 32365) ( Chang et al., 2004 ) and its corresponding ARE mutant plasmid PGL3Basic-Mdm2-ARE-mut obtained by site-directed mutagenesis, pRL-TK control plasmid (Promega, E2241), WT p62 expression vector ( Umemura et al., 2016 ), pCDNA3-Myc3-Nrf2 (Addgene, Plasmid# 21555) ( Furukawa and Xiong, 2005 ) , Flag-p53/pRK5 (Addgene 39237) ( Jiang et al., 2011 ) , NRF2-shRNA (Santa Cruz, sc-37030-SH), Control-shRNA (Santa Cruz, sc-108060) or treated with 10 μM sulforaphane (Millipore Sigma, S4441) or DMSO as control. ..
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